Mycosystema 6: 57-64, 1993


RELATIONSHIP BETWEEN DNA FINGERPRINTS AND PATHOTYPE CHARACTERIATION OF CHINESE RICE BLAST ISOLATES (PYRICULARIA ORYZAE)

ZHU HENG, WANG JU-LIN, LEI CAI-LIN, LING ZHONG-ZHUAN and ZHU LI-HUANG
Institute of Genetics, Academia Sinica, Beijing Institute of Crop Breeding and Cultivation, CAAS, Beijing


ABSTRACT: By selecting from a genomic library of rice blast fungus, Pyricularia oryzae, we have cloned a genome specific and dispersed repeated sequence named POR6, which can be used to fingerprint various kinds of rice blast pathotypes. In this study, 6 isolates from Japan and 26 isolates from northern China werre analyzed using POR6 as a probe. Twentytwo of northern China isolates were assigned to 8 lineages by evaluating the percentage of similar hybridization bands (Sxy) of each pairwise isolates. Some blast isolates maintained in our laboratory were proven to be varied with subsequent culturing in their pathotypes identified by the traditional method. When POR6 was used as a probe, two isolates showed variations in EcoRV digested DNA fingerprints among their asexual generations.